Genotyping of Candida albicans oral strains from healthy individuals by polymorphic microsatellite locus analysis.
نویسندگان
چکیده
Analysis of a polymorphic microsatellite locus was applied to 85 Candida albicans strains from healthy individuals. Comparison with strains from nonhealthy individuals previously analyzed in our laboratory showed an overall similarity, suggesting that all commensal strains have the ability to develop as pathogens.
منابع مشابه
Analysis of microsatellite markers of Candida albicans used for rapid typing.
To obtain a rapid genotyping method of Candida albicans, three polymorphic microsatellite markers were investigated by multiplex PCR. The three loci, called CDC3, EF3, and HIS3, were chosen because they are on different chromosomes so as to improve the chances of finding polymorphisms. One set of primers was designed for each locus, and one primer of each set was dye-labeled to read PCR signals...
متن کاملA highly polymorphic microsatellite for the identification of Candida albicans strains
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 The polymorphism of a new microsatellite locus (CAI) was investigated in a total of 114 C. albicans strains, including 73 independent clinical isolates, multiple isolates from the same patient, isolates from several episodes of recurrent vulvovaginal infections and two reference strains. PCR genotyping was performed automatically, usin...
متن کاملComparative genotyping of Candida albicans bloodstream and nonbloodstream isolates at a polymorphic microsatellite locus.
Molecular typing studies have shown that the predominant form of reproduction of Candida albicans is clonal and that, in a majority of situations, persistent or recurrent infections are due to a unique strain. Characterization of distinct subpopulations and correlation with clinical features may thus be important to understanding the pathogenesis of candidiasis. In a clonal model, a unique poly...
متن کاملCandida albicans: genotyping methods and clade related phenotypic characteristics
Several molecular methods, such as Southern blotting hybridization, Multilocus Sequence Typing, and DNA microsatellite analysis, have been employed to genotype Candida albicans. The genotype analysis allows to group strains in clades, that is, a group composed of one ancestor and its descendants. These genotype studies demonstrate that clades distribution is influenced by geographic area as wel...
متن کاملOral Manifestation and Candida Albicans in Multiple Sclerosis Disease with Different Treatment Modalities
Multiple sclerosis (MS) is a chronic demyelinating disease which is considered to be an autoimmune disease impacting the central nervous system (CNS). Patients with MS may have certain orofacial manifestations and oral microbiological changes as a result of different treatment modalities. Thus, this study aimed to investigate the changes of Candida Albicans in normal oral flora during different...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical microbiology
دوره 41 5 شماره
صفحات -
تاریخ انتشار 2003